receptor thermal protein domain  (MedChemExpress)

Journal: International Journal of Molecular Medicine

Article Title: Remimazolam alleviates myocardial ischemia/reperfusion injury and inflammation via inhibition of the NLRP3/IL-1β pathway in mice

doi: 10.3892/ijmm.2025.5498

Figure Lengend Snippet: Rema alleviates I/R-induced inflammation and activation of NLRP3/IL-1β. (A) Rema inhibited I/R-induced CD68 + cell (red) proliferation and infiltration. The statistical graph showed that Rema inhibited CD68 + cell proliferation induced by I/R injury (n=3). Scale bar, 100 μ m. (B) Effects of Rema on the expression levels of IL-1β (red) were evaluated using immunofluorescence staining. The statistical graph showed that Rema inhibited I/R-induced alterations in IL-1β expression (n=3). Scale bar, 20 μ m. (C) Representative images showing that Rema inhibited I/R-induced alterations in NLRP3 and IL-1β expression. (D) Statistical graphs showing that Rema inhibited the I/R-induced increase in expression of NLRP3 and IL-1β (n=4). *** P<0.001. A.U., arbitrary units; I/R, ischemia/reperfusion; NLRP3, NOD-like receptor thermal protein domain associated protein 3; Rema, remimazolam.

Article Snippet: In addition, the NOD-like receptor thermal protein domain associated protein 3 (NLRP3) inhibitor dapansutrile ( ) (DAPA; 5 μ M; cat. no. HY-17629; MedChemExpress) was used with LPS for treatment for 24 h in an incubator at 37°C.

Techniques: Activation Assay, Expressing, Immunofluorescence, Staining

Journal: World Journal of Gastroenterology

Article Title: Kushenol I combats ulcerative colitis via intestinal barrier preservation and gut microbiota optimization

doi: 10.3748/wjg.v31.i26.105656

Figure Lengend Snippet: Molecular docking and corresponding scores of kushenol I with key targets protein kinase B, p38 mitogen-activated protein kinase, NOD-like receptor thermal protein domain associated protein 3, phosphoinositide 3-kinase, protein kinase B, forkhead box O1, and Toll-like receptor 4, are presented. AKT: Protein kinase B; FOXO1: Forkhead box O1; NF-κB: Nuclear factor kappa B; NLRP3: NOD-like receptor thermal protein domain associated protein 3; p38 MAPK: p38 mitogen-activated protein kinase; PI3K: Phosphoinositide 3-kinase; TLR4: Toll-like receptor 4.

Article Snippet: Antibodies against nuclear factor kappa B (NF-κB) p65, phosphorylated NF-κB p65 (NF-κB p-p65), NOD-like receptor thermal protein domain associated protein 3 (NLRP3), phosphoinositide 3-kinase (PI3K), and phosphorylated PI3K (p-PI3K) were purchased from Cell Signaling Technology Inc. (Danvers, MA, United States).

Techniques:

Journal: World Journal of Gastroenterology

Article Title: Kushenol I combats ulcerative colitis via intestinal barrier preservation and gut microbiota optimization

doi: 10.3748/wjg.v31.i26.105656

Figure Lengend Snippet: Molecular dynamics simulation results of kushenol I interacting with protein kinase B, forkhead box O1, nuclear factor kappa B, NOD-like receptor thermal protein domain associated protein 3, phosphoinositide 3-kinase, p38 mitogen-activated protein kinase, and Toll-like receptor 4. A: Fluctuation curves of hydrogen bond numbers; B: Radius of gyration curves; C: Root mean square deviation curves; D: Root mean square fluctuation curves; E: Free energy landscape. AKT: Protein kinase B; FOXO1: Forkhead box O1; NF-κB: Nuclear factor kappa B; NLRP3: NOD-like receptor thermal protein domain associated protein 3; p38 MAPK: p38 mitogen-activated protein kinase; PI3K: Phosphoinositide 3-kinase; Rg: Gyration radius; RMSD: Root mean square deviation; RMSF: Root mean square deviation; TLR4: Toll-like receptor 4.

Article Snippet: Antibodies against nuclear factor kappa B (NF-κB) p65, phosphorylated NF-κB p65 (NF-κB p-p65), NOD-like receptor thermal protein domain associated protein 3 (NLRP3), phosphoinositide 3-kinase (PI3K), and phosphorylated PI3K (p-PI3K) were purchased from Cell Signaling Technology Inc. (Danvers, MA, United States).

Techniques:

Journal: World Journal of Gastroenterology

Article Title: Kushenol I combats ulcerative colitis via intestinal barrier preservation and gut microbiota optimization

doi: 10.3748/wjg.v31.i26.105656

Figure Lengend Snippet: Effects of kushenol I on the expression of inflammation-related proteins phosphorylated phosphoinositide 3-kinase (PI3K), PI3K, phosphorylated protein kinase B (AKT), AKT, forkhead box O1, interleukin 1β, Toll-like receptor 4, phosphorylated p38 mitogen-activated protein kinase (MAPK), p38 MAPK, nuclear factor kappa B phosphorylated p65, nuclear factor kappa B p65, and NOD-like receptor thermal protein domain associated protein 3 in the colonic tissues of mice with ulcerative colitis. A: Image of a gel showing protein expression; B: Bar graph depicting protein expression. Values are presented as the mean ± standard error of the mean ( n = 3). a P < 0.05. b P < 0.01. c P < 0.001. P compared with the model group. AKT: Protein kinase B; FOXO1: Forkhead box O1; IL-1β: Interleukin 1β; PI3K: Phosphoinositide 3-kinase; p-p38: Phosphorylated p38; p38 MAPK: p38 mitogen-activated protein kinase; p-p65: Phosphorylated p65; p-PI3K: Phosphorylated phosphoinositide 3-kinase; p-AKT: Phosphorylated protein kinase B; NF-κB: Nuclear factor kappa B; NLRP3: NOD-like receptor thermal protein domain associated protein 3; TLR4: Toll-like receptor 4.

Article Snippet: Antibodies against nuclear factor kappa B (NF-κB) p65, phosphorylated NF-κB p65 (NF-κB p-p65), NOD-like receptor thermal protein domain associated protein 3 (NLRP3), phosphoinositide 3-kinase (PI3K), and phosphorylated PI3K (p-PI3K) were purchased from Cell Signaling Technology Inc. (Danvers, MA, United States).

Techniques: Expressing

Journal: World Journal of Gastroenterology

Article Title: Kushenol I combats ulcerative colitis via intestinal barrier preservation and gut microbiota optimization

doi: 10.3748/wjg.v31.i26.105656

Figure Lengend Snippet: Kushenol I preserves the intestinal barrier and optimizes gut microbiota to combat ulcerative colitis. AKT: Protein kinase B; CD: Cluster of differentiation; FOXO1: Forkhead box O1; GSH-PX: Glutathione peroxidase; IL: Interleukin; MDA: Malondialdehyde; MPO: Myeloperoxidase; NF-κB: Nuclear factor kappa B; NLRP3: NOD-like receptor thermal protein domain associated protein 3; PI3K: Phosphoinositide 3-kinase; SOD: Superoxide dismutase; TNF: Tumor necrosis factor; TLR4: Toll-like receptor 4; ZO-1: Zonula occludens-1.

Article Snippet: Antibodies against nuclear factor kappa B (NF-κB) p65, phosphorylated NF-κB p65 (NF-κB p-p65), NOD-like receptor thermal protein domain associated protein 3 (NLRP3), phosphoinositide 3-kinase (PI3K), and phosphorylated PI3K (p-PI3K) were purchased from Cell Signaling Technology Inc. (Danvers, MA, United States).

Techniques: